HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Belsham, G. J. Articles by Lomonossoff, G. P. Search for Related Content PubMed PubMed Citation Articles by Belsham, G. J. Articles by Lomonossoff, G. P. Agricola Articles by Belsham, G. J. Articles by Lomonossoff, G. P.

The Mechanism of Translation of Cowpea Mosaic Virus Middle Component RNA: No Evidence for Internal Initiation from Experiments in an Animal Cell Transient Expression System

¹ AFRC Institute for Animal Health, Pirbright Laboratory, Pirbright, Woking, Surrey GU24 0NF
and² Department of Virus Research, John Innes Institute, John Innes Centre for Plant Science Research, Colney Lane, Norwich NR4 7UH, U.K.

The possibility that internal initiation of translation is responsible for the synthesis of the middle component (M) RNA-encoded 95K protein of cowpea mosaic virus (CPMV) has been investigated by constructing plasmids in which the entire sequence of CPMV M RNA was cloned downstream of a chloramphenicol acetyltransferase gene. Expression of these plasmids in an animal cell expression system revealed that no synthesis of the proteins encoded by the downstream CPMV open reading frame takes place from RNA derived from these constructs under conditions where the internal ribosome entry site of foot-and-mouth disease virus is functional. The results indicate that internal initiation is not responsible for the synthesis of the 95K protein in this system.

Received 18 March 1991; accepted 13 August 1991.

This article has been cited by other articles:

				A. Karetnikov and K. Lehto The RNA2 5' leader of Blackcurrant reversion virus mediates efficient in vivo translation through an internal ribosomal entry site mechanism J. Gen. Virol., January 1, 2007; 88(1): 286 - 297. [Abstract] [Full Text] [PDF]

				M. Niepel and D. R. Gallie Identification and Characterization of the Functional Elements within the Tobacco Etch Virus 5' Leader Required for Cap-Independent Translation J. Virol., November 1, 1999; 73(11): 9080 - 9088. [Abstract] [Full Text] [PDF]