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Cell-free translation and identification of the replicative form of Nudaurelia virus RNA

D. H. du Plessis^1,

¹ Department of Biochemistry and Microbiology, University of Fort Hare, Alice, Ciskei
and² Department of Microbiology, Rhodes University, Grahamstown, South Africa

Larvae of the pine emperor moth, Nudaurelia cytherea capensis, infected with Nudaurelia virus (NV) consistently contained one species of dsRNA. This dsRNA was the correct size to be the replicative form of the NV genome and, in Northern blots, it hybridized with ³²P-end-labelled virion RNA. Other smaller dsRNAs were obtained in a non-reproducible manner but these had no sequences in common with the genomic probe; no dsRNAs were observed in extracts from virus-free larvae. Cell-free translation of NV RNA resulted in the synthesis of only one major polypeptide, of M_r about 71000, which could not be precipitated by an anti-NV serum.

Present address: Biochemistry Section, Veterinary Research Institute, Onderstepoort, South Africa.

Received 22 May 1990; accepted 29 October 1990.