HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Carp, R. I. Articles by Callahan, S. M. Search for Related Content PubMed PubMed Citation Articles by Carp, R. I. Articles by Callahan, S. M. Agricola Articles by Carp, R. I. Articles by Callahan, S. M.

Variation in the characteristics of 10 mouse-passaged scrapie lines derived from five scrapie-positive sheep

NYS Institute for Basic Research in Developmental Disabilities, 1050 Forest Hill Road, Staten Island, New York 10314-6399, U.S.A.

Ten mouse-passaged scrapie lines were initiated from five sheep with clinical scrapie. Of the lines, five were initiated and passaged exclusively in mice with the s7s7 genotype and the remaining five lines were initiated in mice with the p7p7 genotype, with two of these lines subsequently being passaged exclusively in p7p7 mice and two being passaged mainly in p7p7 mice. Lines were passaged three or four times and two parameters were compared: incubation period and the induction of a weight increase during the preclinical period. Considerable variation in the incubation periods was found between the different passage lines at similar passage levels, with a range in s7s7 mice of 113 days to greater than 450 days and a range in p7p7 mice of 219 days to greater than 500 days. All of the lines passaged exclusively in s7s7 mice had shorter incubation periods in this mouse genotype than in p7p7 mice, whereas of the five lines initiated in p7p7 mice, two had shorter incubation periods in p7p7 mouse strains. C57BL mice were used as the indicator strain and most of the lines caused an increase in weight during the preclinical phase of disease compared to control mice injected with normal brain homogenates. For both parameters, incubation period and preclinical weight increase, differences were seen in lines that had identical passage histories, suggesting that an informational molecule separate from host genomic material must specify scrapie strain differences.

Received 29 June 1990; accepted 22 October 1990.

This article has been cited by other articles:

				M. E. Bruce, A. Boyle, S. Cousens, I. McConnell, J. Foster, W. Goldmann, and H. Fraser Strain characterization of natural sheep scrapie and comparison with BSE J. Gen. Virol., March 1, 2002; 83(3): 695 - 704. [Abstract] [Full Text] [PDF]

				R. Carp, H. Meeker, V Caruso, and E Sersen Scrapie strain-specific interactions with endogenous murine leukaemia virus J. Gen. Virol., January 1, 1999; 80(1): 5 - 10. [Abstract]

				R Hecker, A Taraboulos, M Scott, K M Pan, S L Yang, M Torchia, K Jendroska, S J DeArmond, and S B Prusiner Replication of distinct scrapie prion isolates is region specific in brains of transgenic mice and hamsters. Genes & Dev., July 1, 1992; 6(7): 1213 - 1228. [Abstract] [PDF]