HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Shamoon, B.-M. Articles by Mandart, E. Search for Related Content PubMed PubMed Citation Articles by Shamoon, B.-M. Articles by Mandart, E. Agricola Articles by Shamoon, B.-M. Articles by Mandart, E.

Production of polyclonal antibodies against the S and preS2 regions of woodchuck hepatitis virus: lack of detectable low glycosylated preS2 protein (GP33) in sera from infected animals

Elisabeth Mandart

UPR 41 CNRS Recombinaisons Génétiques, Centre Hayem, Hôpital Saint-Louis, 75475 Paris Cedex 10, France

Polyclonal antibodies directed against the preS2 and S domains of the woodchuck hepatitis virus (WHV) envelope proteins were prepared using synthetic peptides and fusion polypeptides as immunogens. They were tested by immunoblotting and immunoprecipitation of infected woodchuck sera and lysates of a eukaryotic cell line expressing WHV envelope proteins. Only one anti-peptide serum directed against the preS2 domain was reactive with WHV envelope proteins, recognizing the preS2 and preS1 proteins by their preS2 epitopes. With recombinant fusion proteins we generated several anti-S sera, which recognized all envelope proteins, and anti-preS2 antisera, which recognized the preS proteins. Results obtained with our antisera showed that sera of infected woodchucks lack the low glycosylated form (GP33) of the preS2 protein, unlike human hepatitis B virus.

Present address: UPR 2420 CNRS, Centre de Génétique Moléculaire, avenue de la Terrasse, 91198 Gif-sur-Yvette, France

Received 4 July 1990; accepted 22 October 1990.

This article has been cited by other articles:

				T. K. Tolle, D. Glebe, M. Linder, D. Linder, S. Schmitt, R. Geyer, and W. H. Gerlich Structure and Glycosylation Patterns of Surface Proteins from Woodchuck Hepatitis Virus J. Virol., December 1, 1998; 72(12): 9978 - 9985. [Abstract] [Full Text] [PDF]