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J Gen Virol 72 (1991), 465-468; DOI 10.1099/0022-1317-72-2-465
© 1991 Society for General Microbiology

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Ambisense segment 3 of rice stripe virus: the first instance of a virus containing two ambisense segments

Tetsuji Kakutani, Yuriko Hayano, Takaharu Hayashi{dagger} and Yuzo Minobe

National Institute of Agrobiological Resources, Tsukuba, Ibaraki 305, Japan

The complete nucleotide sequence of rice stripe virus (RSV) segment 3 shows that it has two open reading frames, one in the viral-complementary sequence, which codes for the nucleocapsid protein, and the other in the viral-sense sequence. The non-coding region between the ambisense genes in RSV segment 3 contains several U and A tracts, as do the ambisense S segments of phleboviruses and uukuviruses. As we have previously shown that RSV segment 4 has an ambisense nature, this is the first instance of a virus containing two ambisense segments.

{dagger} Present address: Kyushu National Agricultural Experiment Station, Chikugo, Fukuoka 833, Japan.

Received 7 August 1990; accepted 29 October 1990.


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E. M. Estabrook, J. Tsai, and B. W. Falk
In vivo transfer of barley stripe mosaic hordeivirus ribonucleotides to the 5' terminus of maize stripe tenuivirus RNAs
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[Abstract] [Full Text] [PDF]




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