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cDNA cloning and nucleotide sequence of the wheat streak mosaic virus capsid protein gene

¹ Plant Pathology Department, University of Florida, Gainesville, Florida 32611,
² Department of Plant Pathology, North Carolina State University, Box 7616, Raleigh, North Carolina 27695, U.S.A.
³ CIAT, AA6713, Cali, Colombia,
⁴ Department of Biology, Washington University, St Louis, Missouri 63130
and⁵ Monsanto Company, 700 Chesterfield Parkway, Chesterfield, Missouri 63198, U.S.A.

The 3'-terminal region of wheat streak mosaic virus (WSMV) genomic RNA was cloned and a cDNA sequence of 1809 nucleotides upstream of the poly(A) tract was determined. The sequence contains a single open reading frame of 1662 nucleotides and a 3' untranslated region of 147 nucleotides. Translation products from WSMV RNA and WSMV cDNA transcripts were immunoprecipitated by WSMV capsid protein antiserum, indicating that the 3'-terminal region of WSMV RNA encodes the capsid protein. Five potential N-terminal capsid protein protease cleavage sites were identified, which would yield proteins ranging from 31.7K to 46.8K. Alignment of the deduced amino acid sequence of the WSMV capsid protein with those of other potyviruses showed significant, but limited, identity as compared to the alignment of two or more aphid-transmitted potyviruses. Although WSMV has characteristics distinct from poty-viruses, because of its particle morphology, translation strategy apparently based on polyprotein processing, the ability to form cytoplasmic cylindrical inclusions and the degree of capsid protein homology with aphidtransmitted potyviruses, it should be considered a member of the potyvirus group.

Received 17 September 1990; accepted 14 November 1990.

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