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Dipartimento di Protezione delle Piante dalle Malattie, Università degli Studi and Centro di Studio del CNR sui Virus e le Virosi delle Colture Mediterranee, Via Amendola 165/A, 70126 Bari, Italy
Nicotiana clevelandii plants were inoculated with cymbidium ringspot tombusvirus RNA synthesized in vitro, after which further passages were made by sap inoculation. During the third passage, low Mr RNA species appeared which had the characteristics of deletion mutants of genomic RNA. Sequence analysis of several of these defective interfering RNAs suggested a possible evolution of smaller from larger molecules. Computer-generated secondary structures of sequences surrounding recombination sites were extensive and stable and these sites occurred in interior or hairpin loops, thus providing a possible explanation for discontinuous RNA transcription and the formation of deletions in genomic RNA.
Permanent address: Agricultural Biotechnology Center, P.O. Box 170, H-2101 Gödöllö, Hungary.
Received 7 August 1990;
accepted 13 November 1990.
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