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Evidence for dissimilar properties of comoviral and picornaviral RNA polymerases

¹ Department of Molecular Biology, Agricultural University, Dreyenlaan 3, 6703 HA Wageningen
and² Department of Virology, Agricultural University, Binnenhaven 11, 6709 PD Wageningen, The Netherlands

The poliovirus RNA polymerase has been synthesized in Spodoptera frugiperda cells by using the baculovirus expression system. Crude sonicates of these cells exhibited an RNA-elongating activity of a synthetic oligo(U) primer with poly(A) or cowpea mosaic virus (CPMV) RNA as a template. A similar polymerase activity was found in extracts of insect cells in which foot-and-mouth disease virus (FMDV) proteins, including the putative polymerase, were produced. The analogous CPMV 87K protein and several of its precursors, synthesized in S. frugiperda cells, did not show any detectable polymerase activity in the same assay under a variety of conditions. The results indicate that, in contrast to the picornaviral polymerases, the CPMV polymerase is unable to function in an oligo(U)-primed polymerase assay.

Received 21 September 1990; accepted 13 November 1990.