HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Everett, R. D. Search for Related Content PubMed PubMed Citation Articles by Everett, R. D. Agricola Articles by Everett, R. D.

Construction and characterization of herpes simplex type 1 viruses without introns in immediate early gene 1

MRC Virology Unit, Church Street, Glasgow G11 5JR, U.K.

Herpes simplex virus type 1 (HSV-1) encodes at least 70 distinct genes in a DNA genome sequence of about 150 kb. In contrast to most cellular genes and those of several other DNA viruses, the overwhelming majority of HSV-1 transcripts are not spliced. One exception is immediate early (IE) gene 1, which contains two introns in the Vmw110 coding region. This study investigated the possibility that IE-1 intron sequences have a role during HSV-1 infection. IE-1 genes lacking the first, second or both introns were constructed by site-directed deletion mutagenesis and recombined into the viral genome. Viruses lacking the IE-1 introns were essentially indistinguishable from the parent virus in terms of growth, particle to p.f.u. ratio or viral polypeptide expression in a variety of cell types. The lack of introns did not affect the time-course or efficiency of expression of Vmw110 either during normal infection or in cycloheximide reversal experiments. In contrast, in transfection assays, the loss of both intron sequences resulted in the elimination of the ability of a plasmid-encoded IE-1 to activate gene expression. These results imply that in certain situations the introns in IE-gene 1 may contribute to the efficient expression of Vmw110 but such an effect is not readily apparent using a recombinant virus in the tissue culture systems tested. In the course of this work a mutant of Vmw110 was fortuitously isolated which had lost the majority of an extremely acidic section of the polypeptide; this mutation appeared to have little effect on Vmw110 function.

Received 31 May 1990; accepted 22 November 1990.

This article has been cited by other articles:

				B. L. Strang and N. D. Stow Circularization of the Herpes Simplex Virus Type 1 Genome upon Lytic Infection J. Virol., October 1, 2005; 79(19): 12487 - 12494. [Abstract] [Full Text] [PDF]

				M. Canning, C. Boutell, J. Parkinson, and R. D. Everett A RING Finger Ubiquitin Ligase Is Protected from Autocatalyzed Ubiquitination and Degradation by Binding to Ubiquitin-specific Protease USP7 J. Biol. Chem., September 10, 2004; 279(37): 38160 - 38168. [Abstract] [Full Text] [PDF]

				R. Hagglund and B. Roizman Role of ICP0 in the Strategy of Conquest of the Host Cell by Herpes Simplex Virus 1 J. Virol., March 1, 2004; 78(5): 2169 - 2178. [Full Text] [PDF]

				A. P. W. Poon, S. J. Silverstein, and B. Roizman An Early Regulatory Function Required in a Cell Type-Dependent Manner Is Expressed by the Genomic but Not the cDNA Copy of the Herpes Simplex Virus 1 Gene Encoding Infected Cell Protein 0 J. Virol., August 28, 2002; 76(19): 9744 - 9755. [Abstract] [Full Text] [PDF]

				T. I. Cornu and J. C. de la Torre RING Finger Z Protein of Lymphocytic Choriomeningitis Virus (LCMV) Inhibits Transcription and RNA Replication of an LCMV S-Segment Minigenome J. Virol., October 1, 2001; 75(19): 9415 - 9426. [Abstract] [Full Text] [PDF]