HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by van Staden, V. Articles by Huismans, H. Search for Related Content PubMed PubMed Citation Articles by van Staden, V. Articles by Huismans, H. Agricola Articles by van Staden, V. Articles by Huismans, H.

A comparison of the genes which encode non-structural protein NS3 of different orbiviruses

Department of Genetics, University of Pretoria, Pretoria 0002, South Africa

The segment 10 (S10) genes of African horsesickness virus (AHSV), Palyam virus and epizootic haemorrhagic disease virus were translated in vitro in a rabbit reticulocyte lysate system. Each of the S10 genes encoded two proteins, NS3 and NS3A, which were shown to be related by peptide mapping. Cloned copies of the S10 genes of two AHSV serotypes (AHSV-3 and AHSV-9) and Palyam virus were sequenced and compared to each other and to the nucleotide sequence of bluetongue virus (BTV) gene S10. Two in-phase ATG translation initiation codons reported for the S10 genes of BTV-10 and BTV-1 were conserved in the S10 genes of AHSV-3, AHSV-9 and Palyam virus, and would be able to initiate synthesis of NS3 and NS3A respectively. Comparison of the amino acid sequences of NS3 of AHSV-3 and AHSV-9 identified two areas of approximately 45 amino acids which displayed high (98%) similarity. One of these areas corresponded to the only region which displayed more than 50% amino acid similarity between NS3 of BTV, AHSV and Palyam virus. This region could represent an important structural or catalytic site of the protein. The overall amino acid similarity outside this conserved region was between 13% and 29%.

Received 22 November 1990; accepted 23 January 1991.

This article has been cited by other articles:

				M. Quan, M. van Vuuren, P. G. Howell, D. Groenewald, and A. J. Guthrie Molecular epidemiology of the African horse sickness virus S10 gene J. Gen. Virol., May 1, 2008; 89(5): 1159 - 1168. [Abstract] [Full Text] [PDF]

				M. van Niekerk, M. Freeman, J. T. Paweska, P. G. Howell, A. J. Guthrie, A. C. Potgieter, V. van Staden, and H. Huismans Variation in the NS3 gene and protein in South African isolates of bluetongue and equine encephalosis viruses J. Gen. Virol., March 1, 2003; 84(3): 581 - 590. [Abstract] [Full Text] [PDF]

				M. van Niekerk, V. van Staden, A. A. van Dijk, and H. Huismans Variation of African horsesickness virus nonstructural protein NS3 in southern Africa J. Gen. Virol., January 1, 2001; 82(1): 149 - 158. [Abstract] [Full Text]

				C. Sailleau, C. Hamblin, J. T. Paweska, and S. Zientara Identification and differentiation of the nine African horse sickness virus serotypes by RT-PCR amplification of the serotype-specific genome segment 2 J. Gen. Virol., March 1, 2000; 81(3): 831 - 837. [Abstract] [Full Text]

				M Yamakawa, M Kubo, and S Furuuchi Molecular analysis of the genome of Chuzan virus, a member of the Palyam serogroup viruses, and its phylogenetic relationships to other orbiviruses J. Gen. Virol., April 1, 1999; 80(4): 937 - 941. [Abstract]

				M Yamakawa, S Furuuchi, and Y Minobe Molecular characterization of double-stranded RNA segments encoding the major capsid proteins of a Palyam serogroup orbivirus that caused an epizootic of congenital abnormalities in cattle J. Gen. Virol., January 1, 1999; 80(1): 205 - 208. [Abstract]