J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 72 (1991), 1099-1104; DOI 10.1099/0022-1317-72-5-1099
© 1991 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Niikura, M.
Right arrow Articles by Mikami, T.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Niikura, M.
Right arrow Articles by Mikami, T.
Agricola
Right arrow Articles by Niikura, M.
Right arrow Articles by Mikami, T.

Expression of the A antigen (gp57-65) of Marek's disease virus by a recombinant baculovirus

Masahiro Niikura1, Yoshiharu Matsuura2, Masakazu Hattori1, Misao Onuma1 and Takeshi Mikami3

1 Department of Epizootiology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060
2 Department of Veterinary Science, National Institute of Health, Tokyo 141
and3 Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo, Tokyo 113, Japan

A recombinant baculovirus expressing the A antigen (A Ag) of Marek's disease virus (MDV) was constructed. In Spodoptera fruiperda (Sf) cells infected with the recombinant virus, A Ag expression was localized to the cell surface. Only a small amount of recombinant A Ag was detected in the culture supernatant of infected Sf cells, but authentic A Ag is mainly secreted into the culture supernatant of MDV-infected chicken embryo fibroblasts. Cell surface-associated recombinant A Ag seemed to be slightly larger than authentic A Ag, whereas the secreted recombinant A Ag seemed to be smaller. The recombinant A Ag was shown to be reactive with the sera of MDV-infected chickens by immunodiffusion studies and ELISA. Sera of chickens immunized with recombinant A Ag formed a precipitin line with the culture supernatant of MDV-infected chicken embryo fibroblasts in an immunodiffusion test. These results indicate that the recombinant A Ag expressed by the recombinant baculovirus retains the antigenic and immunogenic properties of the authentic A Ag.

Received 16 October 1990; accepted 5 February 1991.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1991 by the Society for General Microbiology.