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J Gen Virol 72 (1991), 1187-1190; DOI 10.1099/0022-1317-72-5-1187
© 1991 Society for General Microbiology
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Analysis of the protective effect of the haemagglutinin-neuraminidase protein in Newcastle disease virus infection

Yoshii Nishino1,{dagger}, Masahiro Niikura1, Takahiko Suwa2, Misao Onuma1, Bin Gotoh3, Yoshiyuki Nagai3 and Takeshi Mikami4

1 Department of Epizootiology
2 Department of Comparative Pathology, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060
3 Research Institute for Disease Mechanism and Control, Nagoya University School of Medicine, Nagoya 466
and4 Department of Veterinary Microbiology, Faculty of Agriculture, The University of Tokyo, Tokyo 113, Japan

The role of immune responses to haemagglutinin-neuraminidase (HN) protein in protection against a Newcastle disease virus (NDV) infection was investigated using a recombinant vaccinia virus expressing HN (HN-RVV). Live HN-RVV replicated in chickens and completely protected them from lethal infection with virulent NDV. Inactivated HN-RVV also protected chickens when administered with adjuvant but not when administered without adjuvant. However, large amounts of the inactivated HN-RVV (100-fold excess) without adjuvant provided protection. Specific antibodies against the HN protein of NDV were detected in sera from survivors but not from dying birds. However, the kinetics of antibody responses in chickens inoculated with live HN-RVV and inactivated HN-RVV were considerably different. These results clearly confirm that immune response(s) solely to the HN protein of NDV can provide chickens with protection against NDV challenge, and show that the presence of antibodies to the HN protein correlates significantly with the protection from NDV infection at least in HN-immunized chickens.

{dagger} Present address: Section of Serology, Institute of Immunological Science, Hokkaido University, Kita-ku, Sapporo 060, Japan.

Received 16 October 1990; accepted 31 January 1991.




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Copyright © 1991 by the Society for General Microbiology.