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Biotechnology and Microbiology Division, Wyeth-Ayerst Research, P.O. Box 8299, Philadelphia, Pennsylvania 19101, U.S.A.
The human immunodeficiency virus type 1 (HIV-1) Gag protein was expressed in A549 cells infected with recombinant adenovirus types 4 and 7, each carrying the HIV-1 gag and pro genes. The Gag protein was assembled into enveloped virus-like particles that budded from plasma and vacuolar membranes. The particles, isolated by precipitation and isopycnic density centrifugation, contained both processed and unprocessed Gag-associated proteins.
Received 15 November 1990;
accepted 18 February 1991.
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