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J Gen Virol 72 (1991), 1243-1251; DOI 10.1099/0022-1317-72-6-1243
© 1991 Society for General Microbiology

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Ultrastructural characterization of human immunodeficiency virus type 1 Gag-containing particles assembled in a recombinant adenovirus vector system

Steven K. Vernon, Shridhara Murthy, James Wilhelm, Pranab K. Chanda, Narender Kalyan, Shaw-Guang Lee and Paul P. Hung

Biotechnology and Microbiology Division, Wyeth-Ayerst Research, P.O. Box 8299, Philadelphia, Pennsylvania 19101, U.S.A.

The human immunodeficiency virus type 1 (HIV-1) Gag protein was expressed in A549 cells infected with recombinant adenovirus types 4 and 7, each carrying the HIV-1 gag and pro genes. The Gag protein was assembled into enveloped virus-like particles that budded from plasma and vacuolar membranes. The particles, isolated by precipitation and isopycnic density centrifugation, contained both processed and unprocessed Gag-associated proteins.

Received 15 November 1990; accepted 18 February 1991.


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