J Gen Virol
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 72 (1991), 1309-1315; DOI 10.1099/0022-1317-72-6-1309
© 1991 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Pfleiderer, M.
Right arrow Articles by Siddell, S. G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Pfleiderer, M.
Right arrow Articles by Siddell, S. G.
Agricola
Right arrow Articles by Pfleiderer, M.
Right arrow Articles by Siddell, S. G.

High level transient expression of the murine coronavirus haemagglutinin-esterase

Michael Pfleiderer1,{dagger}, Edward Routledge1,{ddagger}>, Georg Herrler2 and Stuart G. Siddell1

1 Institute of Virology, University of Würzburg, Versbacher Strasse 7, 8700 Würzburg
and2 Institute of Virology, University of Würzburg, Versbacher Strasse 7, 8700 Würzburg, Germany

We have expressed the murine coronavirus haemagglutinin-esterase protein in a vaccinia virus/T7 RNA polymerase system. The levels of expression observed are significantly higher than those found in virus-infected cells. The expressed protein has both receptor-destroying (esterase) and receptor-binding (haemad-sorption) activities. The use of this system will greatly facilitate analysis of the structure-function relationships of this protein.

{dagger} Present address: Immuno Research Centre, Uferstrasse 15, 2304 Orth/Donau, Austria.

{ddagger}> Present address: Department of Pathology, Immunology Division, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, U.K.

Received 4 December 1990; accepted 13 February 1991.


This article has been cited by other articles:


Home page
 J. Virol.Home page
A. Lissenberg, M. M. Vrolijk, A. L. W. van Vliet, M. A. Langereis, J. D. F. de Groot-Mijnes, P. J. M. Rottier, and R. J. de Groot
Luxury at a Cost? Recombinant Mouse Hepatitis Viruses Expressing the Accessory Hemagglutinin Esterase Protein Display Reduced Fitness In Vitro
J. Virol., December 15, 2005; 79(24): 15054 - 15063.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
L. Kazi, A. Lissenberg, R. Watson, R. J. de Groot, and S. R. Weiss
Expression of Hemagglutinin Esterase Protein from Recombinant Mouse Hepatitis Virus Enhances Neurovirulence
J. Virol., December 15, 2005; 79(24): 15064 - 15073.
[Abstract] [Full Text] [PDF]

Home page
 Microbiol. Mol. Biol. Rev.Home page
S. R. Weiss and S. Navas-Martin
Coronavirus Pathogenesis and the Emerging Pathogen Severe Acute Respiratory Syndrome Coronavirus
Microbiol. Mol. Biol. Rev., December 1, 2005; 69(4): 635 - 664.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
G. Regl, A. Kaser, M. Iwersen, H. Schmid, G. Kohla, B. Strobl, U. Vilas, R. Schauer, and R. Vlasak
The Hemagglutinin-Esterase of Mouse Hepatitis Virus Strain S Is a Sialate-4-O-Acetylesterase
J. Virol., June 1, 1999; 73(6): 4721 - 4727.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
A. Klausegger, B. Strobl, G. Regl, A. Kaser, W. Luytjes, and R. Vlasak
Identification of a Coronavirus Hemagglutinin-Esterase with a Substrate Specificity Different from Those of Influenza C Virus and Bovine Coronavirus
J. Virol., May 1, 1999; 73(5): 3737 - 3743.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1991 by the Society for General Microbiology.