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Loss of pseudorabies virus thymidine kinase activity due to a single base mutation and amino acid substitution

Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Madrid, Arzobispo Morcillo 4, 28029 Madrid, Spain

The nucleotide sequences of the coding region of the thymidine kinase (TK) gene of pseudorabies virus strain NIA3 and a TK^- mutant (ATK5) were determined. The coding region of the TK gene consists of 320 codons capable of producing a polypeptide with an M_r of 34979. The mutant expressed an inactive TK polypeptide when translated in vitro; a unique base substitution was detected in the mutant TK modifying amino acid position 13, at which aspartic acid replaces glycine. This modification affects the nucleotide-binding site, thus explaining the expression of an inactive TK polypeptide.

Received 4 December 1990; accepted 18 February 1991.

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