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J Gen Virol 72 (1991), 1515-1520; DOI 10.1099/0022-1317-72-7-1515
© 1991 Society for General Microbiology
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The nucleotide sequence of the infectious cloned DNA components of potato yellow mosaic virus

R. H. A. Coutts1, R. S. Coffin1, E. J. F. Roberts1 and W. D. O. Hamilton2

1 Department of Biology, Imperial College of Science, Technology and Medicine, Prince Consort Road, London SW7 2BB
and2 Applied Plant Technology Laboratory, Agricultural Genetics Company Ltd, Babraham, Cambridge CB2 4AZ, U.K.

The complete nucleotide sequence of a Venezuelan isolate of potato yellow mosaic virus (PYMV) has been determined, showing it to be typical of subgroup I geminiviruses in that it is whitefly-transmitted, has a circular, bipartite ssDNA genome and possesses bidirectionally orientated open reading frames (ORFs). The two genomic components have little sequence similarity apart from a common region of 268 nucleotides (nt) which is almost identical. Analysis of ORFs revealed six potential coding regions encoding proteins of Mr > 10K, four in PYMV A (2593 nt) and two in PYMV B (2547 nt), which are preceded by regulatory transcription elements and have polyadenylation signals present at the ends. Amino acid sequence alignments of PYMV DNA ORF-encoded proteins with those encoded by other previously sequenced geminivirus ORFs show that PYMV is closely related to those geminiviruses isolated from the New World, especially in the putative coat protein gene regions.

Received 23 January 1991; accepted 19 March 1991.




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Copyright © 1991 by the Society for General Microbiology.