HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Zajac, A. J. Articles by Sangar, D. V. Search for Related Content PubMed PubMed Citation Articles by Zajac, A. J. Articles by Sangar, D. V. Agricola Articles by Zajac, A. J. Articles by Sangar, D. V.

Parameters influencing the attachment of hepatitis A virus to a variety of continuous cell lines

Allan J. Zajac

Department of Virology, Wellcome Biotechnology Ltd, Langley Court, Beckenham, Kent BR3 3BS, U.K.

We have investigated the interactions of purified radiolabelled hepatitis A virus (HAV) with a variety of continuous cell lines. Virus labelled either in vitro with radiolabelled iodine or in vivo with radiolabelled uridine bound to cells with similar efficiency. Attachment to BS-C-1 cells was calcium ion-dependent and this correlated with infectivity assay results. The cell tropism of HAV attachment was examined using cell suspensions and confluent cell monolayers at both 4 °C and 37 °C. The maximum level of attachment was observed at 4 °C with cells in suspension, but was severely inhibited by 2% foetal calf serum; these results again correlated with infectivity assays. The components of serum which inhibit attachment have been characterized by gel filtration chromatography, sucrose density gradient analysis, immunoprecipitation and Western blotting. The data show that such components are of high M_r and that the serum glycoprotein, ₂-macroglobulin, can partly mimic the inhibitory effect of whole serum.

Present address: Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, U.K.

Received 20 December 1990; accepted 28 March 1991.

This article has been cited by other articles:

				A. Dotzauer, U. Gebhardt, K. Bieback, U. Göttke, A. Kracke, J. Mages, S. M. Lemon, and A. Vallbracht Hepatitis A Virus-Specific Immunoglobulin A Mediates Infection of Hepatocytes with Hepatitis A Virus via the Asialoglycoprotein Receptor J. Virol., December 1, 2000; 74(23): 10950 - 10957. [Abstract] [Full Text]

				N. E. Bishop and D. A. Anderson Uncoating Kinetics of Hepatitis A Virus Virions and Provirions J. Virol., April 1, 2000; 74(7): 3423 - 3426. [Abstract] [Full Text]