J Gen Virol Faster Access
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 72 (1991), 1755-1760; DOI 10.1099/0022-1317-72-7-1755
© 1991 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Barbone, F. P.
Right arrow Articles by Leibowitz, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Barbone, F. P.
Right arrow Articles by Leibowitz, M. J.
Agricola
Right arrow Articles by Barbone, F. P.
Right arrow Articles by Leibowitz, M. J.

Coupling of killer virus transcription with translation in yeast cell-free extracts

Francis P. Barbone and Michael J. Leibowitz

University of Medicine and Dentistry of New Jersey, Robert Wood Johnson Medical School, Department of Molecular Genetics and Microbiology, 675 Hoes Lane, Piscataway, New Jersey 08854-5635, U.S.A.

The cytoplasmically inherited killer virus of Saccharomyces cerevisiae expresses its dsRNA genome via apparently uncapped viral transcripts produced in the cytoplasm of infected cells. Virions of this naturally temperature-sensitive virus can be added to cell-free translational extracts of uninfected yeast cells resulting in a reaction in which viral transcription and translation are coupled at 15 °C in vitro. In this reaction nucleotides are incorporated into full-length transcripts of the M and L-A dsRNA segments, with lower levels of incorporation into genomic RNA. In addition, incorporation of nucleotides is observed into a smaller RNA species showing no sequence relatedness to M or L-A.

Received 5 November 1990; accepted 25 March 1991.


This article has been cited by other articles:


Home page
 GeneticsHome page
Z. Tallóczy, R. Mazar, D. E. Georgopoulos, F. Ramos, and M. J. Leibowitz
The [KIL-d] Element Specifically Regulates Viral Gene Expression in Yeast
Genetics, June 1, 2000; 155(2): 601 - 609.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1991 by the Society for General Microbiology.