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J Gen Virol 72 (1991), 1811-1817; DOI 10.1099/0022-1317-72-8-1811
© 1991 Society for General Microbiology
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Nuclear Location of the 16K Non-structural Protein of Tobacco Rattle Virus

D. H. Liu{dagger}, D. J. Robinson, G. H. Duncan and B. D. Harrison

Scottish Crop Research Institute, Invergowrie, Dundee DD2 5DA, U.K.

An antiserum, elicited by a synthetic peptide coupled to bovine serum albumin, reacted specifically with the non-structural 16K protein of tobacco rattle virus. The protein was detected in extracts of systemically infected Nicotiana clevelandii leaves, but only in those made with the aid of SDS, urea and 2-mercaptoethanol. Immunogold labelling of ultrathin sections showed that the protein was mainly associated with nuclei, but was also present in the cytoplasm. These observations suggest that the 16K protein binds to macromolecular components of infected cells, especially in nuclei, but do not clarify its function.

{dagger} Present address: Department of Plant Protection, Beijing Agricultural University, Haidian, Beijing 100094, People's Republic of China.

Received 4 February 1991; accepted 29 April 1991.


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