| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Plant Pathology, University of Wisconsin, Madison, Wisconsin 53706
and2 Department of Botany and Plant Pathology, Plant Biology Building, Michigan State University, East Lansing, Michigan 48824-1312, U.S.A.
An examination of the genomic strategy of pea enation mosaic virus (PEMV) RNA 1 has verified strong organizational and sequence relationships between PEMV and the beet western yellows-potato leafroll luteovirus subgroup. Sequence analysis of RNA 1 demonstrated five predominant open reading frames (ORFs). The extreme 5' ORF encodes a 34K product of unknown function. The second ORF encodes an 84K product which overlaps 90% of ORF 1 (in a unique reading frame) and is expressed by internal initiation beginning at the second start codon from the 5' terminus. This protein contains a protease-like motif characteristic of serine- and cysteine-based proteases, suggesting involvement in post-translational processing of viral translation products. The third ORF is characterized by a number of RNA polymerase motifs and a helicase-like motif typical of RNA-dependent RNA polymerases. It overlaps (out of frame) the ORF 2 product and is proposed to be expressed by a frameshift fusion of the ORF 2 and ORF 3 products. The fourth ORF encodes the viral coat protein, and is immediately followed in frame by a 33K ORF thought to represent the aphid transmission subunit of the PEMV virion. Northern blot analysis of polysome-associated RNA suggests that both products are expressed from an 1800 nucleotide subgenomic mRNA, with the 33K product expressed as a read-through fusion with the coat protein monomer.
Received 6 February 1991;
accepted 29 April 1991.
This article has been cited by other articles:
|
|
 |
|
  S. L. Smits, E. J. Snijder, and R. J. de Groot Characterization of a torovirus main proteinase. J. Virol., April 1, 2006; 80(8): 4157 - 4167. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Beier and M. Grimm Misreading of termination codons in eukaryotes by natural nonsense suppressor tRNAs Nucleic Acids Res., December 1, 2001; 29(23): 4767 - 4782. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Mäkinen, K. Mäkeläinen, N. Arshava, T. Tamm, A. Merits, E. Truve, S. Zavriev, and M. Saarma Characterization of VPg and the polyprotein processing of Cocksfoot mottle virus (genus Sobemovirus) J. Gen. Virol., November 1, 2000; 81(11): 2783 - 2789. [Abstract] [Full Text]
|
|
|
|
|
|
T. Tamm and E. Truve Sobemoviruses J. Virol., July 15, 2000; 74(14): 6231 - 6241. [Full Text]
|
|
|
|
|
|
J. S. Skaf, M. H. Schultz, H. Hirata, and G. A. de Zoeten Mutational evidence that the VPg is involved in the replication and not the movement of Pea enation mosaic virus-1 J. Gen. Virol., April 1, 2000; 81(4): 1103 - 1109. [Abstract] [Full Text]
|
|
|
|
 |
|
 E. J. Snijder, A. L. M. Wassenaar, L. C. van Dinten, W. J. M. Spaan, and A. E. Gorbalenya The Arterivirus Nsp4 Protease Is the Prototype of a Novel Group of Chymotrypsin-like Enzymes, the 3C-like Serine Proteases J. Biol. Chem., March 1, 1996; 271(9): 4864 - 4871. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
