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J Gen Virol 72 (1991), 1871-1876; DOI 10.1099/0022-1317-72-8-1871
© 1991 Society for General Microbiology
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Characterization Of Sendai Virus-Induced Human Placental Trophoblast Interferons

George Aboagye-Mathiesen1, Ferenc D. Tóth1,2,, Claus Juhl1, Niels Nørskov-Lauritsen1, Peter M. Petersen1, Vladimir Zachar1,3, and Peter Ebbesen1

The1 Danish Cancer Society, Department of Virus and Cancer, Gustav Wieds Vej 10, DK-8000 Aarhus C, Denmark
2 Institute of Microbiology, Medical University, H-4012 Debrecen, Hungary
and3 Institute of Virology, Slovak Academy of Sciences, Bratislava, Czechoslovakia

Human placental trophoblast cultures produce a mixture of interferons (IFNs) when challenged with Sendai virus. High-performance dye-ligand and immunoaffinity chromatography of a trophoblast IFN (tro-IFN) preparation enabled the isolation of three antigenically distinct IFNs, {alpha}I, {alpha}II1 and beta, with Mrs of 16K, 22K and 24K respectively, by reducing and non-reducing SDS-PAGE. The major IFN, responsible for 75% of the total antiviral activity, was tro-IFN-beta, with the remaining activity being due to tro-IFN-{alpha}I and tro-IFN-{alpha}II1, as determined by an antiviral neutralization test using specific anti-human IFN antibodies. The antiviral activities of the tro-IFNs were stable at pH 2·0 for 24 h and tro-IFN-{alpha}II1 and -beta were shown to be glycoproteins. The three tro-IFNs showed different antiviral activities when assayed on human and bovine cell species; tro-IFN-{alpha}I and -{alpha}II1 protected both human and bovine (MDBK) cells from virus infection, whereas tro-IFN-beta showed a high degree of species specificity, protecting only the human cell types tested.

Received 7 December 1990; accepted 2 April 1991.




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Copyright © 1991 by the Society for General Microbiology.