| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Division of Communicable Diseases, Royal Free Hospital School of Medicine, Rowland Hill Street, Hampstead, London NW3 2QG, U.K.
The heterogeneity of low passage human cytomegalovirus (HCMV) strains was determined by HindIII typing of 28 clinical isolates from transplant patients. These data have shown that, in general, each patient's strain has a unique restriction profile, usually comprising combinations of HindIII sites present in one or more of the tissue culture-adapted strains AD169, Towne and Davis. To map sequence changes in a more refined manner we performed detailed analyses of 33 low passage clinical isolates, including those aforementioned, analysing a sequence within glycoprotein B containing a major neutralizing epitope. A 149 bp sequence containing the epitope (amino acids 608 to 625) was amplified using the polymerase chain reaction, the products were cloned and their DNA sequence was determined. Comparison of the DNA and deduced amino acid sequences with those of HCMV strain AD169 revealed that there was a high degree of conservation of the epitope between the 33 clinical isolates. However 10 of the isolates possessed silent mutations and three isolates contained mutations producing amino acid changes within the neutralizing epitope. The possible functional significance of these changes is discussed.
Received 28 August 1990;
accepted 8 May 1991.
This article has been cited by other articles:
|
|
 |
|
  R. Holtappels, C. O. Simon, M. W. Munks, D. Thomas, P. Deegen, B. Kuhnapfel, T. Daubner, S. F. Emde, J. Podlech, N. K. A. Grzimek, et al. Subdominant CD8 T-Cell Epitopes Account for Protection against Cytomegalovirus Independent of Immunodomination J. Virol., June 15, 2008; 82(12): 5781 - 5796. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Nugent, I. Birch-Machin, K. C. Smith, J. A. Mumford, Z. Swann, J. R. Newton, R. J. Bowden, G. P. Allen, and N. Davis-Poynter Analysis of Equid Herpesvirus 1 Strain Variation Reveals a Point Mutation of the DNA Polymerase Strongly Associated with Neuropathogenic versus Nonneuropathogenic Disease Outbreaks. J. Virol., April 1, 2006; 80(8): 4047 - 4060. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Franti, J.-T. Aubin, G. De Saint-Maur, H. Kosuge, K. Yamanishi, A. Gautheret-Dejean, A. Garbarg-Chenon, J.-M. Huraux, and H. Agut Immune Reactivity of Human Sera to the Glycoprotein B of Human Herpesvirus 7 J. Clin. Microbiol., January 1, 2002; 40(1): 44 - 51. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. M. Kearns, B. Draper, W. Wipat, A. J. L. Turner, J. Wheeler, R. Freeman, J. Harwood, F. K. Gould, J. H. Dark, L. Schaade, et al. LightCycler-Based Quantitative PCR for Detection of Cytomegalovirus in Blood, Urine, and Respiratory Samples J. Clin. Microbiol., June 1, 2001; 39(6): 2364 - 2365. [Full Text] [PDF]
|
|
|
|
 |
|
 V. C. Emery and P. D. Griffiths Prediction of cytomegalovirus load and resistance patterns after antiviral chemotherapy PNAS, June 14, 2000; (2000) 140123497. [Abstract] [Full Text]
|
|
|
|
|
|
R. N. Jones, M. L. Neale, B. Beattie, D. Westmoreland, and J. D. Fox Development and Application of a PCR-Based Method Including an Internal Control for Diagnosis of Congenital Cytomegalovirus Infection J. Clin. Microbiol., January 1, 2000; 38(1): 1 - 6. [Abstract] [Full Text]
|
|
|
|
 |
|
 A. Speckner, D. Glykofrydes, M. Ohlin, and M. Mach Antigenic domain 1 of human cytomegalovirus glycoprotein B induces a multitude of different antibodies which, when combined, results in incomplete virus neutralization J. Gen. Virol., August 1, 1999; 80(8): 2183 - 2191. [Abstract] [Full Text]
|
|
|
|
 |
|
 V. C. Emery, A. V. Cope, E. F. Bowen, D. Gor, and P. D. Griffiths The Dynamics of Human Cytomegalovirus Replication In Vivo J. Exp. Med., July 19, 1999; 190(2): 177 - 182. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Zweygberg Wirgart, M. Brytting, A. Linde, B. Wahren, and L. Grillner Sequence Variation within Three Important Cytomegalovirus Gene Regions in Isolates from Four Different Patient Populations J. Clin. Microbiol., December 1, 1998; 36(12): 3662 - 3669. [Abstract] [Full Text]
|
|
|
|
|
|
V. C. Emery and P. D. Griffiths Prediction of cytomegalovirus load and resistance patterns after antiviral chemotherapy PNAS, July 5, 2000; 97(14): 8039 - 8044. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
