HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Cavanagh, H. M. A. Articles by Spibey, N. Search for Related Content PubMed PubMed Citation Articles by Cavanagh, H. M. A. Articles by Spibey, N. Agricola Articles by Cavanagh, H. M. A. Articles by Spibey, N.

A mutant of canine adenovirus type 2 with a duplication of the E1a region exhibits altered expression of early region 4

Heather M. A. Cavanagh

Department of Veterinary Pathology, University of Glasgow Veterinary School, Bearsden Road, Glasgow, U.K.

The genomic DNA of a vaccine strain of canine adenovirus type 2 (Vaxitas; ICI Tasman) has been shown to contain two copies of the E1a region, the second being at the far right end of the genome. DNA sequence analysis of the right terminal 2.8 kbp of this vaccine strain showed that numerous point mutations have occurred in the second copy, which would preclude the synthesis of any functional products. However, expression vectors in which the E1a promoter from the right terminus were linked to the chloramphenicol acetyltransferase gene showed that the promoter was fully functional. Furthermore, the activity of the reiterated E1a promoter was considerably greater than that of the normal E4 promoter. This dramatic change in the regulation of E4 expression may be an important factor in determining the altered host cell specificity displayed by this vaccine strain virus.

Present address: Department of Neurovirology, University of Glasgow, Institute of Neurology, Southern General Hospital, Glasgow, U.K.

Received 31 October 1990; accepted 21 May 1991.