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A comparison of WIN 51711 and R 78206 as stabilizers of poliovirus virions and procapsids

¹ Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Laarbeeklaan 103, B-1090 Brussels
and² Janssen Research Foundation, Turnhoutseweg 30, B-2340 Beerse, Belgium

The thermal denaturation of poliovirus virions and procapsids in the 42 to 48 °C range was studied using N- and H-specific monoclonal antibodies. The half-life of the N antigen of Mahoney and Sabin 1 virions was extended 50- to 250-fold by either 10 µM WIN 51711 or R 78206. The minimum concentrations required for full stabilization at 46 °C (1.0 µM for WIN 51711, 0.2 µM for R 78206) were independent of the strain or serotype of the virus; 30 to 60 molecules of stabilizer per virion were required for full protection. R 78206 was the most efficient stabilizer of Mahoney procapsids; the half-life of the N-specific epitopes of these particles at 44 °C was extended from less than 1 min to 1 day.

Received 8 March 1991; accepted 29 May 1991.

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