J Gen Virol Email Content Delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 73 (1992), 173-178; DOI 10.1099/0022-1317-73-1-173
© 1992 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Galun, E.
Right arrow Articles by Blum, H. E.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Galun, E.
Right arrow Articles by Blum, H. E.
Agricola
Right arrow Articles by Galun, E.
Right arrow Articles by Blum, H. E.

Human non-hepatocytes support hepadnaviral replication and virion production

Eithan Galun1,{dagger}, Wolf-Bernhard Offensperger2, Fritz von Weizsäcker1, Silke Offensperger2, Jack R. Wands1 and Hubert E. Blum1

1 Massachusetts General Hospital, Harvard Medical School, Molecular Hepatology Laboratory, MGH Cancer Center, Charlestown, Massachusetts 02129, U.S.A.
and2 Department of Medicine, University of Freiburg, 7800 Freiburg, Germany

The competence of non-hepatocytes to support hepatitis B virus (HBV) gene expression and replication was studied by transient transfection of various human cell lines with a head-to-tail dimer of HBV DNA. Independent of their neuroectodermal, mesenchymal or epithelial origin, all non-hepatocyte cell lines tested synthesized and secreted hepatitis B surface antigen (HBsAg) and hepatitis B core/e antigen (HBc/eAg). Further analyses of two of these cell lines (LS 180 and COLO 320) identified the two major HBV transcripts of 3.6 and 2.2/2.4 kb length, respectively. LS 180 cells were permissive for HBV and duck hepatitis B virus (DHBV) DNA replication and secretion of infectious virions. COLO 320 cells also supported HBV DNA replication, but did not appear to export complete viral particles. These findings provide direct evidence that both HBV and DHBV can replicate in non-hepatic tumour cell lines, one of which is shown also to produce infectious virions.

{dagger} Present address: Liver Unit, Department of Medicine, Hadassah University Hospital, Ein Karem, PO Box 12000, Jerusalem 91120, Israel.

Received 21 June 1991; accepted 11 September 1991.




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1992 by the Society for General Microbiology.