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Proteins of bovine herpesvirus type 4 released into the culture medium of productively infected cells: identification of a 135K glycoprotein involved in viral attachment

Jean Dubuisson

Igor Koromyslov^>

Department of Virology-Immunology, Faculty of Veterinary Medicine, University of Liège, Institut de Chimie (B6), 4000 Liège, Belgium

Three bovine herpesvirus type 4 (BHV-4) proteins released into the culture medium of infected cells were identified, with M_r values of 135K, 16K and 14.5K. Among these three proteins, two were precipitated by the monoclonal antibodies characterized in this work. One is a glycoprotein of 135K (gp8) which does not seem to be involved in BHV-4 neutralization. Moreover, this 135K glycoprotein adsorbed onto uninfected susceptible cells. The attachment of gp8 to cells was totally inhibited by the prior adsorption of unlabelled viral proteins. Moreover, anti-gp8 monoclonal antibodies were effective in inhibiting the adsorption of gp8. These results indicate that gp8 could be involved in BHV-4 attachment.

Present address: Department of Molecular Microbiology, Washington University School of Medicine, 660 South Euclid Avenue (Box 8230), St Louis, Missouri 63110-1093, U.S.A.

^> Present address: Zvénigorodskoye Road 5, Institute for the Control of Veterinary Products, 123022 Moscow, U.S.S.R.

Received 30 May 1991; accepted 12 September 1991.

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