| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
1 Department of Microbiology, University of Hong Kong, Pathology Building, Queen Mary Hospital Compound, Hong Kong
2 NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR
and3 ‘Green Valleys’, Goodleigh, Barnstaple, Devon EX32 7NH, U.K.
Complementary DNAs were synthesized from the envelope protein genes of two isolates of dengue virus (TH-36 and TH-Sman, previously suggested as possible dengue virus type 5 and dengue virus type 6 respectively) and amplified by the polymerase chain reaction using sense and antisense primers designed from conserved dengue virus gene sequences. The amplified cDNA clones were sequenced in both directions by double-stranded dideoxynucleotide sequencing. Alignment with published dengue virus sequences enabled us to assign these viruses accurately to classified serotypes, confirming that TH-36 and TH-Sman are strains of dengue virus type 2 and dengue virus type 1 respectively. Amino acid changes between the proteins encoded by these two isolates and strains of their respective serotypes may account for the significant antigenic differences observed during previous serological typing of these viruses. Moreover, sequence alignment of flavivirus envelope proteins revealed a hypervariable region, within which members of the dengue and tick-borne virus antigenic complexes show unique peptide sequences. This type-specific hypervariable domain may be useful as a genetic marker for typing dengue and tick-borne flaviviruses.
Received 19 June 1991;
accepted 29 August 1991.
This article has been cited by other articles:
|
|
 |
|
  M. LAILLE and C. ROCHE COMPARISON OF DENGUE-1 VIRUS ENVELOPE GLYCOPROTEIN GENE SEQUENCES FROM FRENCH POLYNESIA Am J Trop Med Hyg, October 1, 2004; 71(4): 478 - 484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. S. Gritsun, T. V. Frolova, A. I. Zhankov, M. Armesto, S. L. Turner, M. P. Frolova, V. V. Pogodina, V. A. Lashkevich, and E. A. Gould Characterization of a Siberian Virus Isolated from a Patient with Progressive Chronic Tick-Borne Encephalitis J. Virol., December 6, 2002; 77(1): 25 - 36. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Hayasaka, L. Ivanov, G. N. Leonova, A. Goto, K. Yoshii, T. Mizutani, H. Kariwa, and I. Takashima Distribution and characterization of tick-borne encephalitis viruses from Siberia and far-eastern Asia J. Gen. Virol., June 1, 2001; 82(6): 1319 - 1328. [Abstract] [Full Text]
|
|
|
|
|
|
E. Wang, H. Ni, R. Xu, A. D. T. Barrett, S. J. Watowich, D. J. Gubler, and S. C. Weaver Evolutionary Relationships of Endemic/Epidemic and Sylvatic Dengue Viruses J. Virol., April 1, 2000; 74(7): 3227 - 3234. [Abstract] [Full Text]
|
|
|
|
|
|
M Ecker, S. Allison, T Meixner, and F. Heinz Sequence analysis and genetic classification of tick-borne encephalitis viruses from Europe and Asia J. Gen. Virol., January 1, 1999; 80(1): 179 - 185. [Abstract]
|
|
|
|
|
|
R. Gualano, M. Pryor, M. Cauchi, P. Wright, and A. Davidson Identification of a major determinant of mouse neurovirulence of dengue virus type 2 using stably cloned genomic-length cDNA J. Gen. Virol., March 1, 1998; 79(3): 437 - 446. [Abstract]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
