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1 Department of Internal Medicine, Division of Clinical Immunology
and2 Renal Transplantation Unit, University Hospital, Oostersingel 59, 9713 EZ Groningen, The Netherlands
and3 Institut für Klinische und Molekulare Virologie, Universität Erlangen-Nürnberg, Loschgestrasse 7, D-8520 Erlangen, Germany
During an active infection with human cytomegalovirus (HCMV), viral antigen is consistently present in peripheral blood leukocytes. Two monoclonal antibodies (MAbs), CMV-C10 and CMV-C11, are commonly used in the HCMV antigenaemia assay to detect these cells in the peripheral blood of patients suspected of having an active HCMV infection. We demonstrate that the viral antigen detected by these MAbs is the viral structural protein pp65 and not an immediate early antigen as previously reported. Furthermore, significantly fewer leukocytes were found to be positive with MAbs specific for immediate early antigens.
Received 16 April 1992;
accepted 10 July 1992.
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