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J Gen Virol 73 (1992), 3231-3234; DOI 10.1099/0022-1317-73-12-3231
© 1992 Society for General Microbiology
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Induction of a novel protein kinase in pupae of the silkworm Bombyx mori after infection with nuclear polyhedrosis virus

Evgeny A. Zemskov, Elena B. Abramova and Victor S. Mikhailov

N. K. Koltzov Institute of Developmental Biology, Russian Academy of Sciences, Vavilov street 26, Moscow 117808, Russia

Protein kinases induced by Bombyx mori nuclear polyhedrosis virus in pupae of the silkworm B. mori were examined by activity gel analysis using phosvitin as a protein substrate. The method involved PAGE of the soluble fraction from pupae under native conditions and in the presence of SDS, followed by in situ renaturation of proteins and recovery of protein kinase activity in the intact gel. A novel protein kinase able to phosphorylate phosvitin was detected in the infected pupae from 2 days post-infection. This enzyme was not present in uninfected silkworms at any stage of the pupal period. The novel kinase activity was found by SDS-PAGE to be associated with a single polypeptide with an apparent Mr of 50K. However, on electrophoresis under native conditions its activity was associated with a set of polypeptides with similar but not identical electrophoretic mobilities. Microheterogeneity of the catalytically active polypeptides suggests that the virus-induced protein kinase undergoes post-translational modification during the course of infection.

Received 16 March 1992; accepted 10 August 1992.




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Copyright © 1992 by the Society for General Microbiology.