HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Jackson, R. J. Articles by Bults, H. G. Search for Related Content PubMed PubMed Citation Articles by Jackson, R. J. Articles by Bults, H. G. Agricola Articles by Jackson, R. J. Articles by Bults, H. G.

A myxoma virus intergenic transient dominant selection vector

Cooperative Research Centre for Biological Control of Vertebrate Pest Populations, CSIRO Division of Wildlife and Ecology, PO Box 84, Lyneham 2602, Canberra A.C.T., Australia

The purpose of this study was to construct an intergenic transfer vector which can be used for the generation of recombinant myxoma viruses (MVs) expressing a foreign gene insert. Recombinant MVs expressing the Escherichia coli lacZ gene were constructed in vitro by transfection of MV-infected rabbit cells with a transfer expression vector, and isolated under growth conditions selecting for transient expression of the E. coli gpt gene. The effect of inserting foreign DNA sequences between the viral thymidine kinase gene and open reading frame MF8a upon the transcription of these genes was investigated.

Received 8 July 1992; accepted 27 August 1992.

This article has been cited by other articles:

				J. Bárcena, M. Morales, B. Vázquez, J. A. Boga, F. Parra, J. Lucientes, A. Pagès-Manté, J. M. Sánchez-Vizcaíno, R. Blasco, and J. M. Torres Horizontal Transmissible Protection against Myxomatosis and Rabbit Hemorrhagic Disease by Using a Recombinant Myxoma Virus J. Virol., February 1, 2000; 74(3): 1114 - 1123. [Abstract] [Full Text]