HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Pise, C. A. Articles by Holland, C. A. Search for Related Content PubMed PubMed Citation Articles by Pise, C. A. Articles by Holland, C. A. Agricola Articles by Pise, C. A. Articles by Holland, C. A.

Human immunodeficiency virus type 1-infected HL-60 cells are capable of both monocytic and granulocytic differentiation

¹ The Center for Virology, Immunology and Infectious Disease Research, Children's Research Institute, Children's National Medical Center, 111 Michigan Avenue, NW, Washington, D.C. 20010
and² Department of Molecular Genetics and Microbiology
and³ Department of Pediatrics, University of Massachusetts Medical Center, 55 Lake Avenue North, Worcester, Massachusetts 01655, U.S.A.

We have used the human myelomonocytic cell line HL-60 as a model system to determine whether human immunodeficiency virus type 1 (HIV-1) infection affects differentiation of myeloid progenitor cells. HL-60 cells were infected with three HIV-1 isolates (IIIB, NL4-3 and PM213). HIV-1 antigen expression and cytopathicity in HL-60 cells infected with each of the three isolates was delayed by approximately 15 days as compared to those in the prototypic T cell line, H9. Chronically infected HL-60 cells and clonal lines derived from them were treated with dimethyl formamide (DMF) and induced to differentiate into granulocytes. Approximately the same percentage of these cells as of DMF-treated, uninfected HL-60 cells differentiated. Superoxide production by infected and uninfected DMF-induced cells was similar. Likewise, approximately the same percentage of cells in infected and uninfected cultures became adherent and were positive for non-specific esterase when monocytic differentiation was induced. The data demonstrate that HL-60 cells infected with HIV-1 are capable of morphological and functional granulocytic and monocytic differentiation.

Received 11 June 1992; accepted 28 August 1992.

This article has been cited by other articles:

				E. Cassol, M. Alfano, P. Biswas, and G. Poli Monocyte-derived macrophages and myeloid cell lines as targets of HIV-1 replication and persistence J. Leukoc. Biol., November 1, 2006; 80(5): 1018 - 1030. [Abstract] [Full Text] [PDF]

				L. Bolduc, B. Labrecque, M. Cordeau, M. Blanchette, and B. Chabot Dimethyl Sulfoxide Affects the Selection of Splice Sites J. Biol. Chem., May 11, 2001; 276(20): 17597 - 17602. [Abstract] [Full Text] [PDF]