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1 Department of Virology (Section of Fundamental Virology), University of Amsterdam, Academic Medical Centre, Meibergdreef 15, 1105 AZ, Amsterdam
and2 Department of Dermatology, University Hospital Leiden, Rijnsburgerweg 10, 2333 AA Leiden, The Netherlands
DNA well suited for polymerase chain reaction (PCR) amplification was purified from archival Papanicolaou smears. The detection of a wide range of human papillomavirus (HPV) types was made possible using a HPV-specific consensus primer pair, and typing was conveniently done by direct sequence analysis of the PCR product. The method could be of unique value in longitudinal and cross-sectional studies aimed at answering a number of fundamental pathological and epidemiological questions regarding HPV infection of the genital tract.
Received 19 May 1992;
accepted 10 August 1992.
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