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In vitro infection of normal human keratinocytes by human papillomavirus type 1 followed by amplification of the viral genome in reconstructed epidermis

¹ Department of Molecular Biology, Université Libre de Bruxelles, 67 rue des Chevaux, 1640 Rhode Saint Genèse
² Experimental Dermatology Unit, Université Libre de Bruxelles, Hôpital Erasme, 1070 Brussels, Belgium
and³ Molecular Oncology Unit, CNRS URA1160, Institut Pasteur de Lille, 59019 Lille, France

Primary cultures of normal human keratinocytes were inoculated in vitro with human papillomavirus type 1 (HPV-1), the agent responsible for deep plantar warts. Upon transfer to dead de-epidermized dermis and growth at the air-liquid interface, keratinocytes reconstituted a pseudoepidermis. Under these highly differentiating conditions, HPV-1 DNA amplification was found to take place in the reconstructed epidermis, being detectable from 7 days after the transfer and persisting for at least 10 days thereafter. The extent of keratinocyte differentiation may be insufficient to allow a complete HPV infectious cycle.

Received 24 April 1992; accepted 10 August 1992.