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Department of Pathology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QP, U.K.
Murine gammaherpesvirus 68 (MHV-68) is able to persist in spleen cells of infected mice. To determine the cell type harbouring persistent virus, spleen cells from infected animals were separated into immunoglobulin (Ig)-positive (B cell-enriched), Ig-negative (T cell-enriched) and plastic-adherent (macrophage-enriched) fractions. These cells were co-cultivated with permissive BHK-21 cells in an infectious centre assay. The consistent recovery and enrichment of infectious centres in the Ig-positive fraction clearly demonstrates that B cells are a major site of virus persistence/latency. This observation indicates that MHV-68 is biologically similar to Epstein—Barr virus and other members of the B cell lymphotropic gammaherpesvirus 1 subgroup.
Present address: Division of Medical Virology, Institute of Medical and Veterinary Science, Frome Road, Adelaide, SA 5000, Australia.
Received 29 June 1992;
accepted 5 August 1992.
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