| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
Medical Research Council Virology Unit, Institute of Virology, Church Street, Glasgow G11 5JR, U.K.
We constructed recombinant viruses based on the herpes simplex virus type 1 mutant tsK which individually were able to express the products of four viral DNA replication genes (UL5, UL8, UL9 and UL52) in the absence of any of the other proteins required for viral DNA synthesis. These viruses were used in immunofluorescence experiments to investigate the cellular localization of the four replication proteins expressed. The results demonstrated that all three components of the viral helicase-primase complex (UL5, UL8 and UL52 proteins) must be co-expressed to allow their efficient localization to the nucleus. Since the UL5 and UL52 proteins together form a complex which is enzymatically indistinguishable from a complex formed from all three proteins, a possible role of the UL8 protein may be in facilitating nuclear uptake. The UL9 protein (origin-binding protein) efficiently entered the cell nucleus when expressed alone. Both UL9 protein and the tripartite helicase-primase complex exhibited patterns of fluorescence which resembled the ‘pre-replicative sites’ described previously.
Received 2 September 1991;
accepted 20 November 1991.
This article has been cited by other articles:
|
|
 |
|
  C. M. Livingston, N. A. DeLuca, D. E. Wilkinson, and S. K. Weller Oligomerization of ICP4 and Rearrangement of Heat Shock Proteins May Be Important for Herpes Simplex Virus Type 1 Prereplicative Site Formation J. Virol., July 1, 2008; 82(13): 6324 - 6336. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, C. M. Livingston, S. D. Carrington-Lawrence, P. Bai, and S. K. Weller A Mutation in the Human Herpes Simplex Virus Type 1 UL52 Zinc Finger Motif Results in Defective Primase Activity but Can Recruit Viral Polymerase and Support Viral Replication Efficiently J. Virol., August 15, 2007; 81(16): 8742 - 8751. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Chen, S. D. Carrington-Lawrence, P. Bai, and S. K. Weller Mutations in the Putative Zinc-Binding Motif of UL52 Demonstrate a Complex Interdependence between the UL5 and UL52 Subunits of the Human Herpes Simplex Virus Type 1 Helicase/Primase Complex J. Virol., July 15, 2005; 79(14): 9088 - 9096. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. L. Uprichard and D. M. Knipe Conformational Changes in the Herpes Simplex Virus ICP8 DNA-Binding Protein Coincident with Assembly in Viral Replication Structures J. Virol., July 1, 2003; 77(13): 7467 - 7476. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Zhou and S. D. Hayward Nuclear Localization of CBF1 Is Regulated by Interactions with the SMRT Corepressor Complex Mol. Cell. Biol., September 15, 2001; 21(18): 6222 - 6232. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Constantin and M. S. Dodson Two-hybrid analysis of the interaction between the UL52 and UL8 subunits of the herpes simplex virus type 1 helicase-primase J. Gen. Virol., September 1, 1999; 80(9): 2411 - 2415. [Abstract] [Full Text]
|
|
|
|
 |
|
 N. Biswas and S. K. Weller A Mutation in the C-terminal Putative Zn2+ Finger Motif of UL52 Severely Affects the Biochemical Activities of the HSV-1 Helicase-Primase Subcomplex J. Biol. Chem., March 19, 1999; 274(12): 8068 - 8076. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z. Gao, A. Krithivas, J. E. Finan, O. J. Semmes, S. Zhou, Y. Wang, and S. D. Hayward The Epstein-Barr Virus Lytic Transactivator Zta Interacts with the Helicase-Primase Replication Proteins J. Virol., November 1, 1998; 72(11): 8559 - 8567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. C. Spector, L. Liang, H. Giordano, M. Sivaraja, and M. G. Peterson Inhibition of Herpes Simplex Virus Replication by a 2-Amino Thiazole via Interactions with the Helicase Component of the UL5-UL8-UL52 Complex J. Virol., September 1, 1998; 72(9): 6979 - 6987. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Schmitt and G. Keil Characterization of the bovine herpesvirus 1 UL8 gene and gene products J. Gen. Virol., January 1, 1998; 79(1): 133 - 141. [Abstract]
|
|
|
|
|
|
D. J. Tenney, A. K. Sheaffer, W. W. Hurlburt, M. Bifano, and R. K. Hamatake Sequence-dependent Primer Synthesis by the Herpes Simplex Virus Helicase-Primase Complex J. Biol. Chem., April 21, 1995; 270(16): 9129 - 9136. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Biswas and S. K. Weller The UL5 and UL52 Subunits of the Herpes Simplex Virus Type 1 Helicase-Primase Subcomplex Exhibit a Complex Interdependence for DNA Binding J. Biol. Chem., May 11, 2001; 276(20): 17610 - 17619. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
