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Poliovirus antigenic hybrids simultaneously expressing antigenic determinants from all three serotypes

Andrew D. Murdin

Michael G. Murray^>

Department of Microbiology, State University of New York, Stony Brook, New York 11794, U.S.A.

We have constructed six hybrid polioviruses (PVs) modified to express PV type 2 and type 3 antigenic determinants on a PV type 1 (Mahoney) capsid. The hybrids were modified in neutralizing antigenic site (NAg) I and/or NAgII. They were viable, but impaired for growth in comparison to PV1 (Mahoney). Some hybrids modified to express type 2 and type 3 NAgI determinants simultaneously displayed some type 2 but no type 3 antigenicity (in addition to type 1 antigenicity associated with other antigenic sites). Hybrids modified to express a type 2 NAgI determinant and a type 3 NAgII determinant, or vice versa, displayed antigenic characteristics of all three serotypes, although expression of the modified NAgII determinant was weak. We conclude that it is possible to construct a viable hybrid PV simultaneously modified in NAgI and NAgII which expresses antigenic determinants of all three serotypes.

Present address: Connaught Laboratories Ltd, 1755 Steeles Avenue West, Willowdale, Ontario, Canada M2R 3T4.

^> Present address: Schering-Plough Research, 60 Orange Street, Bloomfield, New Jersey 07003, U.S.A.

Received 28 August 1991; accepted 14 November 1991.

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