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1 Department of Biological Science and Technology, Science University of Tokyo, Noda, Chiba 278
and2 Department of Molecular Genetics, National Institute of Genetics, Mishima, Shizuoka 411, Japan
A new transfection system for influenza virus was developed using the clone 76 cell line, in which the viral RNA polymerase and nucleoprotein (NP) genes can be expressed in response to dexamethasone. Ribonucleoprotein (RNP) complexes were reconstituted by expressing proteins from a chimeric NS-chloramphenicol acetyltransferase (CAT) RNA consisting of the full-length negative-strand RNA of the CAT gene positioned between the 5'- and 3'-terminal sequences of influenza virus RNA segment 8, and purifying NP from an NP gene-expressing Escherichia coli strain. When the reconstituted RNP was transfected into clone 76 cells, CAT was produced only when the synthesis of the three RNA polymerase subunits and NP was induced by treatment with dexamethasone.
Present address: Department of Biomolecular Engineering, Faculty of Bioscience and Technology, Tokyo Institute of Technology, Midori-ku, Yokohama, Kanagawa 227, Japan.
Received 16 October 1991;
accepted 29 January 1992.
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