HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Riederer, M. A. Articles by Jiricny, J. Search for Related Content PubMed PubMed Citation Articles by Riederer, M. A. Articles by Jiricny, J. Agricola Articles by Riederer, M. A. Articles by Jiricny, J.

The mode of cauliflower mosaic virus propagation in the plant allows rapid amplification of viable mutant strains

Markus A. Riederer

Nigel H. Grimsley^>

Josef Jiricny

Friedrich Miescher-Institut, P.O. Box 2543, CH-4002 Basel, Switzerland

We inoculated the leaves of turnip plants (Brassica campestris spp. rapa cv. Just Right) with two cauliflower mosaic viruses (CaMVs) with different small mutations in a dispensable region of the viral genome, and followed the spread of the virus infection through the plant. Surprisingly, analysis of viral DNA in single primary chlorotic lesions revealed the presence of both mutants. In contrast, the secondary chlorotic lesions and systemically infected leaves contained virus molecules of either one or the other type only. Infection of plants with different ratios of the two reporter viruses showed that this ratio is not conserved during systemic virus spread. Infection with CaMV DNA in the form of heteroduplexes containing a single mismatched base pair, in which each strand carried a distinct diagnostic marker, provided us with evidence that the mismatch was subjected to a repair process in the host plant.

Present address: Department of Biochemistry, Medical Center, University of Stanford, California 94305-5307, U.S.A.

^> Present address: Laboratoire de Biologie Moléculaire INRA/CNRS, Auzeville BP27, 31326 Castanet-Tolosan Cedex, France.

Present address: IRBM, Via Pontina km 30600, 00124 Pomezia (Roma), Italy.

Received 2 December 1991; accepted 19 February 1992.

This article has been cited by other articles:

				B. Hohn and H. Puchta Gene therapy in plants PNAS, July 20, 1999; 96(15): 8321 - 8323. [Full Text] [PDF]

				M. M. Pooggin, T. Hohn, and J. Futterer Forced Evolution Reveals the Importance of Short Open Reading Frame A and Secondary Structure in the Cauliflower Mosaic Virus 35S RNA Leader J. Virol., May 1, 1998; 72(5): 4157 - 4169. [Abstract] [Full Text] [PDF]