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Rice hoja blanca virus genome characterization and expression in vitro

Gabriel Macaya^1,

¹ Institut Jacques Monod, 2 Place Jussieu-Tour 43, 75251 Paris Cedex 05, France
and² Centro Internacional de Agricultura Tropical (CIAT), Apartado Aereo 67-13, Cali, Colombia

No information exists on the organization and mechanisms of expression of the genome of rice hoja blanca virus (RHBV), a member of the tenuivirus group, but here we describe the first steps in its characterization. RHBV contains four ssRNA and three dsRNA species, the sizes of which were estimated by native and denaturing gel electrophoresis. Hybridization analyses using ³²P-labelled riboprobes of viral and viral complementary polarities showed that unequal amounts of the two polarities of at least the smallest RNA are present in the virion, and indicated that the dsRNA species contain the same information as the ssRNA species of corresponding size. Total RHBV RNA directs the synthesis of two major proteins of 23K and 21K in vitro. RNA3 directs the synthesis of a 23K protein designated NS3, and RNA4 of a 21K protein designated NS4. The NS4 protein corresponds to the non-structural protein that accumulates in RHBV-infected rice tissue. The nucleocapsid protein is not translated from either total RHBV RNA or any individual RHBV RNA in vitro.

Permanent address: Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San José, Costa Rica.

Received 7 January 1992; accepted 25 February 1992.

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