J Gen Virol Try Microbiology Online
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

J Gen Virol 73 (1992), 1457-1464; DOI 10.1099/0022-1317-73-6-1457
© 1992 Society for General Microbiology
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Ramirez, B.-C.
Right arrow Articles by Haenni, A.-L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Ramirez, B.-C.
Right arrow Articles by Haenni, A.-L.
Agricola
Right arrow Articles by Ramirez, B.-C.
Right arrow Articles by Haenni, A.-L.

Rice hoja blanca virus genome characterization and expression in vitro

Bertha-Cecilia Ramirez1, Gabriel Macaya1,{dagger}, Lee A. Calvert2 and Anne-Lise Haenni1

1 Institut Jacques Monod, 2 Place Jussieu-Tour 43, 75251 Paris Cedex 05, France
and2 Centro Internacional de Agricultura Tropical (CIAT), Apartado Aereo 67-13, Cali, Colombia

No information exists on the organization and mechanisms of expression of the genome of rice hoja blanca virus (RHBV), a member of the tenuivirus group, but here we describe the first steps in its characterization. RHBV contains four ssRNA and three dsRNA species, the sizes of which were estimated by native and denaturing gel electrophoresis. Hybridization analyses using 32P-labelled riboprobes of viral and viral complementary polarities showed that unequal amounts of the two polarities of at least the smallest RNA are present in the virion, and indicated that the dsRNA species contain the same information as the ssRNA species of corresponding size. Total RHBV RNA directs the synthesis of two major proteins of 23K and 21K in vitro. RNA3 directs the synthesis of a 23K protein designated NS3, and RNA4 of a 21K protein designated NS4. The NS4 protein corresponds to the non-structural protein that accumulates in RHBV-infected rice tissue. The nucleocapsid protein is not translated from either total RHBV RNA or any individual RHBV RNA in vitro.

{dagger} Permanent address: Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San José, Costa Rica.

Received 7 January 1992; accepted 25 February 1992.


This article has been cited by other articles:


Home page
 J. Gen. Virol.Home page
E. Schnettler, H. Hemmes, R. Goldbach, and M. Prins
The NS3 protein of rice hoja blanca virus suppresses RNA silencing in mammalian cells
J. Gen. Virol., January 1, 2008; 89(1): 336 - 340.
[Abstract] [Full Text] [PDF]

Home page
 RNAHome page
H. Hemmes, L. Lakatos, R. Goldbach, J. Burgyan, and M. Prins
The NS3 protein of Rice hoja blanca tenuivirus suppresses RNA silencing in plant and insect hosts by efficiently binding both siRNAs and miRNAs
RNA, July 1, 2007; 13(7): 1079 - 1089.
[Abstract] [Full Text] [PDF]

Home page
 J. Virol.Home page
E. Bucher, T. Sijen, P. de Haan, R. Goldbach, and M. Prins
Negative-Strand Tospoviruses and Tenuiviruses Carry a Gene for a Suppressor of Gene Silencing at Analogous Genomic Positions
J. Virol., December 20, 2002; 77(2): 1329 - 1336.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
INT J SYST EVOL MICROBIOL MICROBIOLOGY J GEN VIROL
J MED MICROBIOL ALL SGM JOURNALS
Copyright © 1992 by the Society for General Microbiology.