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Functional mapping of the rev-responsive element of human immunodeficiency virus type 2 (HIV-2): influence of HIV-2 envelope-encoding sequences on HIV-1 gp120 expression in the presence or absence of Rev

Michael Emerman

Unité d'Oncologie Virale (CNRS UA1157), Institut Pasteur, 28 rue du Dr Roux 75724 Paris, Cedex 15, France

The human immunodeficiency virus type 1 (HIV-1) regulatory protein Rev stimulates expression of structural viral proteins via a target response element (RRE) located within gag-pol and env mRNAs. To analyse the HIV-2 Rev trans-activation effect on the expression of the envelope protein, we cloned a functionally active HIV-2 rev cDNA and showed that it contained four exons. Using transient expression assays, we mapped a 353 bp RRE fragment within the env gene of HIV-2 on which both HIV-1 and HIV-2 Rev could act. Interestingly, smaller fragments suppressed the use of additional splice sites within the env gene and caused envelope protein expression independent of Rev.

Present address: Program in Molecular Medicine, Fred Hutchinson Cancer Research Center, Seattle, Washington 98104, U.S.A.

Received 6 November 1991; accepted 28 February 1992.