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J Gen Virol 73 (1992), 1825-1828; DOI 10.1099/0022-1317-73-7-1825
© 1992 Society for General Microbiology
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Identification of two novel human immunodeficiency virus type 1 splice acceptor sites in infected T cell lines

Jacqueline Smith1, Ahmed Azad2 and Nicholas Deacon1

1 AIDS Molecular Biology Laboratory, Macfarlane Burnet Centre for Medical Research, Fairfield Hospital, Fairfield, Victoria 3078
and2 Biomolecular Research Institute, Parkville, Victoria 3052, Australia

The regulatory and accessory proteins of human immunodeficiency virus type 1 (HIV-1) are produced from singly or multiply spliced mRNAs. We have used HIV-1-specific oligonucleotide primer pairs in a polymerase chain reaction procedure on RNA from lymphocyte cell lines infected with HIV-1228200. The amplified products were analysed by hybridization with splice junction-specific oligonucleotide probes to determine splice donor/splice acceptor combination utilization, subcloned into a plasmid vector and the nucleotide sequences were obtained. Two novel splice acceptor sites have been identified.

Received 6 November 1991; accepted 26 February 1992.


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Copyright © 1992 by the Society for General Microbiology.