J Gen Virol
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J Gen Virol 73 (1992), 1855-1859; DOI 10.1099/0022-1317-73-7-1855
© 1992 Society for General Microbiology
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In vitro reconstitution of active influenza virus ribonucleoprotein complexes using viral proteins purified from infected cells

J. Martín1, C. Albo1, J. Ortín2, J. A. Melero1 and A. Portela1

1 Instituto de Salud Carlos III, Centro Nacional de Microbiología, Servicio de Biología Molecular, 28220 Majadahonda, Madrid
and2 Centro Nacional de Biotecnología (CSIC) and Centro de Biología Molecular (CSIC-UAM), Cantoblanco 28049, Madrid, Spain

A procedure to obtain RNA-free preparations containing the nucleoprotein (NP) and polymerase (P) proteins from influenza virus-infected cell extracts has been developed. The influenza virus P proteins present in these preparations copied small synthetic RNA molecules derived from plasmid sequences. In addition, RNA molecules encapsidated with the NP and P proteins were amplified and packaged into virus particles when transfected into influenza virus-infected cells. Thus, the preparations of NP and P proteins display features similar to those isolated from purified influenza virions, and represent an alternative for the preparation of active influenza virus P protein.

Received 20 December 1991; accepted 23 March 1992.


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Copyright © 1992 by the Society for General Microbiology.