HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Munis, J. R. Articles by Richman, D. D. Search for Related Content PubMed PubMed Citation Articles by Munis, J. R. Articles by Richman, D. D. Agricola Articles by Munis, J. R. Articles by Richman, D. D.

Ordered appearance of human immunodeficiency virus type 1 nucleic acids following high multiplicity infection of macrophages

Douglas D. Richman

Departments of Medicine and Pathology, University of California, San Diego and Veterans Affairs Medical Center, San Diego, California 92161, U.S.A.

The order of appearance of human immunodeficiency virus type 1 (HIV-1) nucleic acids was examined in monocyte-derived macrophages following a high multiplicity infection with macrophage-tropic virus. Using the polymerase chain reaction, viral DNA was first detected 2 h after infection and continued to accumulate over the next 24 h. Transcripts representing tat, rev and nef splicing were detected by 24 h, and transcripts representing env splicing were detected by 48 h after infection. Coincident with the appearance of env transcripts, new synthesis of cellular and extracellular p24 antigen began, multinucleated giant cells formed and progeny infectious virus emerged. This analytical system provides a foundation for further studies on the effects of antiviral agents and cellular factors on the replication cycle of HIV-1 in non-transformed, primary monocyte-derived macrophages.

Present address: University of California, San Diego, Departments of Pathology and Medicine 0679, 9500 Gilman Drive, La Jolla, California 92093-0679, U.S.A.

Received 29 August 1991; accepted 25 March 1992.

This article has been cited by other articles:

				E. A. Acheampong, Z. Parveen, L. W. Muthoga, M. Kalayeh, M. Mukhtar, and R. J. Pomerantz Human Immunodeficiency Virus Type 1 Nef Potently Induces Apoptosis in Primary Human Brain Microvascular Endothelial Cells via the Activation of Caspases J. Virol., April 1, 2005; 79(7): 4257 - 4269. [Abstract] [Full Text] [PDF]

				S. Sonza, H. P. Mutimer, K. O'Brien, P. Ellery, J. L. Howard, J. H. Axelrod, N. J. Deacon, S. M. Crowe, and D. F. J. Purcell Selectively Reduced tat mRNA Heralds the Decline in Productive Human Immunodeficiency Virus Type 1 Infection in Monocyte-Derived Macrophages J. Virol., November 13, 2002; 76(24): 12611 - 12621. [Abstract] [Full Text] [PDF]

				M. Rigourd, C. Ehresmann, M. A. Parniak, B. Ehresmann, and R. Marquet Primer Unblocking and Rescue of DNA Synthesis by Azidothymidine (AZT)-resistant HIV-1 Reverse Transcriptase. COMPARISON BETWEEN INITIATION AND ELONGATION OF REVERSE TRANSCRIPTION AND BETWEEN (-) AND (+) STRAND DNA SYNTHESIS J. Biol. Chem., May 17, 2002; 277(21): 18611 - 18618. [Abstract] [Full Text] [PDF]

				E. T. Sawai, A. S. Baur, B. M. Peterlin, J. A. Levy, and C. Cheng-Mayer A Conserved Domain and Membrane Targeting of Nef from HIV and SIV Are Required for Association with a Cellular Serine Kinase Activity J. Biol. Chem., June 23, 1995; 270(25): 15307 - 15314. [Abstract] [Full Text] [PDF]