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Identification and characterization of pseudo-recombinants of red clover mottle comovirus

¹ Swedish University of Agricultural Sciences, Department of Plant and Forest Protection, P.O. Box 7044, S-75007 Uppsala, Sweden
and² Department of Virus Research, John Innes Institute and AFRC Institute of Plant Science Research, Colney Lane, Norwich NR4 7UH, U.K.

cDNA clones specific for the two genomic RNAs of strain O of the comovirus red clover mottle virus (RCMV) were constructed. Using these clones, in conjunction with clones specific for RNAs of RCMV strain S, local lesion isolates containing reciprocal pseudo-recombinants between strains S and O were identified. Investigation of the biological properties of these pseudo-recombinants showed that the ability of RCMV to infect Chenopodium quinoa is determined by B RNA. The results also suggest that both RNAs are involved in symptom formation in Pisum sativum. Analysis of the strain O clones enabled the sequences at the 3' ends of both genomic RNAs of strain O to be determined. Comparison of these sequences with the corresponding region of the strain S RNAs suggests that the 3' terminal sequences critical for replicase recognition may lie somewhat upstream of the poly(A) tract.

Received 23 January 1992; accepted 29 April 1992.

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