| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
|
|
|
|||||||
|
|||||||||
AFRC Institute for Animal Health, Compton Laboratory, Compton, Nr Newbury, Berkshire RG16 0NN, U.K.
The regions of the fusion protein of respiratory syncytial virus (RSV) that react with neutralizing, fusion-inhibiting and highly protective bovine and murine monoclonal antibodies (MAbs) were mapped by two methods: (i) competitive binding assays and (ii) production and analysis of antibody-escape mutants. Competitive binding assays with 16 murine and 10 bovine MAbs identified 11 antigenic sites on the fusion (F) protein, many of which overlapped extensively, and indicated that cattle, a natural host for RSV, and mice recognize similar epitopes. Neutralizing MAbs identified four sites, two of which were also fusion-inhibiting and highly protective in mice. The pattern of reactivity of antibody-escape mutants with the MAbs confirmed the mapping of the protective epitopes deduced from competitive binding assays. A comparison of the biological properties of MAbs to the F protein indicated that protection against RSV infection correlated with fusion inhibition rather than neutralization titre or complement-dependent lysis of virus-infected cells.
Received 20 March 1992;
accepted 11 May 1992.
This article has been cited by other articles:
|
|
 |
|
  R. Lizundia, K.-S. Sauter, G. Taylor, and D. Werling Host species-specific usage of the TLR4-LPS receptor complex Innate Immunity, August 1, 2008; 14(4): 223 - 231. [Abstract] [PDF]
|
|
|
|
 |
|
 S.-J. Wu, A. Schmidt, E. J. Beil, N. D. Day, P. J. Branigan, C. Liu, L. L. Gutshall, C. Palomo, J. Furze, G. Taylor, et al. Characterization of the epitope for anti-human respiratory syncytial virus F protein monoclonal antibody 101F using synthetic peptides and genetic approaches J. Gen. Virol., October 1, 2007; 88(10): 2719 - 2723. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-F. Valarcher, J. Furze, S. G. Wyld, R. Cook, G. Zimmer, G. Herrler, and G. Taylor Bovine respiratory syncytial virus lacking the virokinin or with a mutation in furin cleavage site RA(R/K)R109 induces less pulmonary inflammation without impeding the induction of protective immunity in calves J. Gen. Virol., June 1, 2006; 87(6): 1659 - 1667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. W. McL. Rixon, G. Brown, J. T. Murray, and R. J. Sugrue The respiratory syncytial virus small hydrophobic protein is phosphorylated via a mitogen-activated protein kinase p38-dependent tyrosine kinase activity during virus infection J. Gen. Virol., February 1, 2005; 86(2): 375 - 384. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. M. Crump, B. Bruun, S. Bell, L. E. Pomeranz, T. Minson, and H. M. Browne Alphaherpesvirus glycoprotein M causes the relocalization of plasma membrane proteins J. Gen. Virol., December 1, 2004; 85(12): 3517 - 3527. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Shi, D. F. Lappin, and R. M. Elliott Mapping the Golgi Targeting and Retention Signal of Bunyamwera Virus Glycoproteins J. Virol., October 1, 2004; 78(19): 10793 - 10802. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. W. McL. Rixon, G. Brown, J. Aitken, T. McDonald, S. Graham, and R. J. Sugrue The small hydrophobic (SH) protein accumulates within lipid-raft structures of the Golgi complex during respiratory syncytial virus infection J. Gen. Virol., May 1, 2004; 85(5): 1153 - 1165. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J.-F. Valarcher, J. Furze, S. Wyld, R. Cook, K.-K. Conzelmann, and G. Taylor Role of Alpha/Beta Interferons in the Attenuation and Immunogenicity of Recombinant Bovine Respiratory Syncytial Viruses Lacking NS Proteins J. Virol., August 1, 2003; 77(15): 8426 - 8439. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Brown, H. W. McL. Rixon, and R. J. Sugrue Respiratory syncytial virus assembly occurs in GM1-rich regions of the host-cell membrane and alters the cellular distribution of tyrosine phosphorylated caveolin-1 J. Gen. Virol., August 1, 2002; 83(8): 1841 - 1850. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Werling, R. A. Collins, G. Taylor, and C. J. Howard Cytokine responses of bovine dendritic cells and T cells following exposure to live or inactivated bovine respiratory syncytial virus J. Leukoc. Biol., August 1, 2002; 72(2): 297 - 304. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Brown, J. Aitken, H. W. McL. Rixon, and R. J. Sugrue Caveolin-1 is incorporated into mature respiratory syncytial virus particles during virus assembly on the surface of virus-infected cells J. Gen. Virol., March 1, 2002; 83(3): 611 - 621. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. W. McL. Rixon, C. Brown, G. Brown, and R. J. Sugrue Multiple glycosylated forms of the respiratory syncytial virus fusion protein are expressed in virus-infected cells J. Gen. Virol., January 1, 2002; 83(1): 61 - 66. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Sugrue, C. Brown, G. Brown, J. Aitken, and H. W. McL. Rixon Furin cleavage of the respiratory syncytial virus fusion protein is not a requirement for its transport to the surface of virus-infected cells J. Gen. Virol., June 1, 2001; 82(6): 1375 - 1386. [Abstract] [Full Text]
|
|
|
|
 |
|
 H. BELANGER, N. FLEYSH, S. COX, G. BARTMAN, D. DEKA, M. TRUDEL, H. KOPROWSKI, and V. YUSIBOV Human respiratory syncytial virus vaccine antigen produced in plants FASEB J, November 1, 2000; 14(14): 2323 - 2328. [Abstract] [Full Text]
|
|
|
|
|
|
G. P. Bembridge, J. A. Lopez, R. Bustos, J. A. Melero, R. Cook, H. Mason, and G. Taylor Priming with a Secreted Form of the Fusion Protein of Respiratory Syncytial Virus (RSV) Promotes Interleukin-4 (IL-4) and IL-5 Production but Not Pulmonary Eosinophilia following RSV Challenge J. Virol., December 1, 1999; 73(12): 10086 - 10094. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. P. Bembridge, R. Garcia-Beato, J. A. Lopez, J. A. Melero, and G. Taylor Subcellular Site of Expression and Route of Vaccination Influence Pulmonary Eosinophilia Following Respiratory Syncytial Virus Challenge in BALB/c Mice Sensitized to the Attachment G Protein J. Immunol., September 1, 1998; 161(5): 2473 - 2480. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Chargelegue, O. E. Obeid, S.-C. Hsu, M. D. Shaw, A. N. Denbury, G. Taylor, and M. W. Steward A Peptide Mimic of a Protective Epitope of Respiratory Syncytial Virus Selected from a Combinatorial Library Induces Virus-Neutralizing Antibodies and Reduces Viral Load In Vivo J. Virol., March 1, 1998; 72(3): 2040 - 2046. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| INT J SYST EVOL MICROBIOL | MICROBIOLOGY | J GEN VIROL |
| J MED MICROBIOL | ALL SGM JOURNALS | |
