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The effects of poly(I).poly(C₁₂U) and interferon on the multiplication of a mammalian type C retrovirus in human cells

¹ U245 INSERM, 184 rue du Fg. St. Antoine, 75012 Paris
and² UPR043 CNRS, 1 avenue Claude Vellefaux, 75010 Paris, France

Poly(I).poly(C₁₂U) or interferon treatment inhibited multiplication of the xenotropic baboon type C endogenous retrovirus M7 in chronically infected human AV3-M7 cells, as determined by a reverse transcriptase (RT) assay and electron microscopy. Furthermore, this polynucleotide induced 2'5' oligoadenylate (2'5'A) synthetase activity. In contrast to interferon (IFN), poly(I).poly(C₁₂U) did not give rise to the appearance of a trapping phenomenon observable by electron microscopy. When AV3-M7 cells were treated simultaneously with poly(I).poly(C₁₂U) and anti-IFN-/ antibodies, the induction of 2'5'A synthetase was abolished without any alteration of the inhibitory effect of RT activity. Taken together, these results suggest that different mechanisms are used by poly(I).poly(C₁₂U) and IFN in blocking type C retrovirus multiplication.

Received 17 February 1992; accepted 4 May 1992.