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1 Division of Virology, School of Pathological Sciences, Medical School, Framlington Place, Newcastle upon Tyne NE4 2HH
2 Department of Veterinary Pathology, University of Liverpool, Leahurst, Neston, Wirral, Merseyside L64 7TE
and3 Department of Biochemistry, University of Liverpool, P.O. Box 147, Liverpool L69 3BX, U.K.
We report the localization of three monoclonal antibody (MAb) binding sites in the capsid protein of feline calicivirus. Gene fragments were generated by restriction enzyme digestion or the polymerase chain reaction, and expressed as 
-galactosidase fusion proteins in Escherichia coli. These chimeric molecules were screened using three MAbs. A non-neutralizing MAb recognized a region within 36 amino acids of the C terminus. Two neutralizing MAbs bound to a different region of 37 amino acids in the centre of the protein. Comparative sequence analysis shows this area to be the major variable region of the capsid protein.
Present address: Department of Biological Sciences, South Road, Durham DH1 3LE, U.K.
Received 25 February 1992;
accepted 27 May 1992.
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