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J Gen Virol 74 (1993), 103-107; DOI 10.1099/0022-1317-74-1-103
© 1993 Society for General Microbiology
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Immunocytochemical characterization of p24, a baculovirus capsid-associated protein

Gregory M. Wolgamot, Christian H. Gross, Rebecca L. Q. Russell and George F. Rohrmann

Department of Agricultural Chemistry, Agricultural and Life Sciences 1007, Oregon State University, Corvallis, Oregon 97331-7301, U.S.A.

An open reading frame (ORF 1) located upstream of the polyhedron envelope protein gene in the Orgyia pseudotsugata multinucleocapsid nuclear polyhedrosis virus (OpMNPV) genome was cloned in-frame into a trpE bacterial expression vector. The fusion protein produced by this construct was used for the preparation of a monospecific antiserum. Western blot analysis of extracts from OpMNPV-infected Lymantria dispar cells and Autographa californica NPV (AcMNPV)-infected Spodoptera frugiperda cells detected a 24K protein late in infection. This antiserum also reacted with a 24K protein in preparations of budded and polyhedra-derived virus from OpMNPV and AcMNPV. The 24K protein was not N-glycosylated. Immunoelectron microscopy confirmed that the OpMNPV p24 is associated with nucleocapsids of budded and polyhedra-derived virions.

Received 13 August 1992; accepted 25 September 1992.


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Copyright © 1993 by the Society for General Microbiology.