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J Gen Virol 74 (1993), 109-114; DOI 10.1099/0022-1317-74-1-109
© 1993 Society for General Microbiology
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Sequencing and antigenic studies of a Norwegian virus isolated from encephalomyelitic sheep confirm the existence of louping ill virus outside Great Britain and Ireland

George F. Gao1, Wen R. Jiang1, Mohammed H. Hussain2, Karunakarannair Venugopal1, Tamara S. Gritsun1, Hugh W. Reid2 and Ernest A. Gould1

1 NERC Institute of Virology and Environmental Microbiology, Mansfield Road, Oxford OX1 3SR
and2 Moredun Research Institute, 408 Gilmerton Road, Edinburgh EH17 7JH, U.K.

We have carried out an antigenic analysis and nucleotide sequence comparison of the envelope glycoprotein of recognized louping ill virus strains isolated from Scotland with that of a Norwegian virus known to cause encephalomyelitis in sheep. Monoclonal antibodies with defined specificity for the louping ill virus envelope glycoprotein failed to distinguish between the Norwegian virus and prototype louping ill virus in indirect immunofluorescence, haemagglutination inhibition and neutralization tests. Nucleotide sequencing of the envelope glycoprotein and alignment of the deduced amino acid sequence with other known sequences revealed that the Norwegian virus closely resembles (> 95% identity for nucleotide and > 98% identity for amino acid sequences) louping ill virus. Maximum variation in identities among four strains of louping ill virus were 4.4% and 1.8% respectively for nucleotide and amino acid alignments. We conclude that sheep encephalomyelitis in Norway is caused by louping ill virus. These results imply that other viruses present in Europe and known to cause encephalitis/encephalomyelitis of sheep could be caused by louping ill virus.

Received 17 June 1992; accepted 28 September 1992.


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[Abstract] [Full Text] [PDF]


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