HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS		QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Smits, P. H. M. Articles by ter Schegget, J. Search for Related Content PubMed PubMed Citation Articles by Smits, P. H. M. Articles by ter Schegget, J. Agricola Articles by Smits, P. H. M. Articles by ter Schegget, J.

Regulation of human papillomavirus type 16 (HPV-16) transcription by loci on the short arm of chromosome 11 is mediated by the TATAAAA motif of the HPV-16 promoter

Department of Medical Microbiology, Section Fundamental Virology, Academic Medical Centre, University of Amsterdam, Room L1-158, Meibergdreef 15, 1105 AZ Amsterdam, The Netherlands

The human papillomavirus type 16 (HPV-16) enhancer-promoter is virtually inactive in normal human diploid fibroblasts, but active in human fibroblasts with a deletion in the short arm of one chromosome 11 (del-11 cells). Since the HPV-16 enhancer with the simian virus 40 promoter is active in both cell types, the target for chromosome 11-regulated HPV-expression is likely to be located in the HPV-16 early promoter region (nucleotides 57 to 112). We show here that DNA-protein complexes formed with an HPV-16 promoter fragment are quantitatively different in del-11 cell and diploid cell extracts. This quantitative difference detected in band shift experiments disappeared upon mutation of the HPV-16 TATAAAA box to TATTTAT. This mutation also strongly reduced the activity of the HPV-16 enhancer-promoter in del-11 cells. These results indicate that TATA-binding proteins are involved in the chromosome 11-mediated regulation of HPV-16 gene expression.

Received 3 July 1992; accepted 14 September 1992.

This article has been cited by other articles:

				K. W. Vance, M. S. Campo, and I. M. Morgan A Novel Silencer Element in the Bovine Papillomavirus Type 4 Promoter Represses the Transcriptional Response to Papillomavirus E2 Protein J. Virol., March 15, 2001; 75(6): 2829 - 2838. [Abstract] [Full Text]

				K. W. Vance, M. S. Campo, and I. M. Morgan An Enhanced Epithelial Response of a Papillomavirus Promoter to Transcriptional Activators J. Biol. Chem., September 24, 1999; 274(39): 27839 - 27844. [Abstract] [Full Text] [PDF]